![]() This type of medium is often a complex growth medium. ![]() tryptic soy agar (TSA) or Luria broth (LB) is used to grow a wide variety of non-fastidious bacteria. Synthetic (also called chemically defined) growth media are formulated to an exactly defined chemical composition.Complex growth media contain ingredients whose exact chemical composition is unknown (e.g.Growth media can be categorized based on their chemical constituents, or the purpose for which they are used. Deeps are used for several different types of differential metabolic tests (e.g., the gelatinase test, which you will perform in Lab 5). Agar plates can be used to separate mixtures of bacteria and to observe colony characteristics of different species of bacteria (you will perform an experiment in this lab to illustrate this). Agar slants are commonly used to generate stocks of bacteria. ![]() They are also the method of choice for growing large quantities of bacteria. Figure 2.1.1: Solid growth media formsīroths can be used to determine growth patterns in a liquid medium, and for certain types of inoculations and metabolic tests that you will be doing later in the semester. Agar plates are made by pouring melted agar into a petri dish. To make agar deeps or agar slants, melted agar is poured into a test tube and then allowed to solidify vertically (agar deep), or at a slant (agar slant). Solid growth media is used in the following forms: agar plates, agar slants, and agar deeps. It is used as a solidification agent because it (1) is not broken down by bacteria, (2) contains no nutrients that can be used by bacteria and (3) melts at high temperatures, and yet is solid at temperatures used for most bacterial growth. Solid growth media usually contains agar, which is a mixture of polysaccharides derived from red algae. There are two commonly used physical forms of growth media: liquid media and solid growth media. Growth media contain a variety of nutrients necessary to sustain the growth of microorganisms. To study bacteria and other microorganisms, it is necessary to grow them in controlled conditions in the laboratory.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |